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KMID : 0861020170320030063
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Korea Journal of Herbology
2017 Volume.32 No. 3 p.63 ~ p.69
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Inhibitory Activity of Advanced Glycation Endproducts (AGEs) Formation and Antioxidant Activity of Processed Korean Medicines
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Lee Ah-Reum
Kwon Oh-Jun
Choi Joon-Young
Roh Seong-Soo
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Abstract
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Objectives： Advanced glycation end product (AGEs) is combine formation of glucose and protein. AGEs and reactive oxygen species are potential therapeutic targets for the various disease such as diabetic complications, renal injury, skin damage. The aim of this study was investigated the AGEs inhibitory activity and antioxidant activity of water extracts from 40 Korean medicines and 5 heating-processed Korean medicines.
Methods: AGEs formation inhibitory activities of Korean medicines measured using bovine serum albumin (BSA), glucose, and fructose. Then, five effective Korean medicines were selected and heated with 30% ethanol. The AGEs inhibitory activities of heated Korean medicine were measured compared with not-heated Korean medicines. The antioxidant activities were evaluated through radical scavenging assays using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2`-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radicals. Furthermore, we examined total phenol and flavonoids contents.
Results: Scutellariae Radix, Corni Fructus, Persimmon Fruit, Paeoniae Radix, Mori Folium respectively reduced AGEs production. Morever, heating-processed Scutellariae Radix has AGEs inhibitory activities better than not-processed Scutellariae Radix. Heating- processed Scutellariae Radix scavenged DPPH and ABTS effectively and IC50 of DPPH and ABTS radical scavenging activity of Heat processed Scutellariae Radix were 15.47±0.26 ㎍/㎖ and 12.07±1.23 ㎍/㎖. It caused heat processing methods of Scutellariae Radix up regulated total phenol and flavonoids contents (26.68±0.01 to 46.15±0.10, 20.30±0.38 to 64.20 ±0.52).
Conclusion: It has AGEs inhibitory activities that 20 kind of medicinal plants of 40 medicinal plants. Especially, heat processed Scutellariae Radix has excellent AGEs inhibitory activities and antioxidant effect.
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KEYWORD
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Medicinal plants, processing method, antioxidant activity, advanced glycation end products (AGEs)
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